UPDATE: New information has been added to the Applications tab

Silica-based Amino Column with Superior Durability

FEATURES

Innovative water resistance compared to conventional silica-based amino columns

Low bleed column for LC-MS with minimal ligand hydrolysis

Sharp peak separation from monosaccharides to oligosaccharides

Applicable not only in normal-phase mode but also in anion-exchange mode

High separation efficiency with 3μm particles

Silica-based aminopropyl columns are crucial for separating carbohydrates in normal-phase chromatography. However, traditional aminopropyl columns have a significant drawback: the ligand undergoes hydrolysis when exposed to water, leading to rapid retention loss and column bleeding.

The newly developed Unison UK-Amino stationary phase addresses these challenges with an innovative design that combines aminopropyl functionality with exceptional water durability. This design ensures robust analytical performance, high resolution due to 3μm particle size, and minimal noise levels in LC-MS and LC-ELSD applications.

Furthermore, it offers high versatility by enabling the separation of various non-carbohydrate compounds in both non-aqueous and aqueous normal-phase modes.

FEATURES

[Remarkable Applications]

[Carbohydrates]

[Other]

>>>See all Unison UK-Amino Application Database <<<

Glycan analysis of Fetuin (Column comparison) (Glycosolutions Corp., USA)

Normal-phase separation of creatinine and purine metabolites (ACCTA, Inc., USA)

LC-MS/MS analysis of monosaccharides (Indiana University, USA)

Sonoporation-Enhanced Delivery of STING Agonist Induced Robust Immune Modulation and Tumor Regression

Efficient production of the rare sugar L-gulose using a wheat-bran culture extract of Penicillium sp. KU-1

Effect of Inoculated Lactic Acid Fermentation on the Fermentable Saccharides and Polyols, Polyphenols and Antioxidant Activity Changes in Wheat Sourdough

Rethinking Remdesivir: Synthesis, Antiviral Activity, and Pharmacokinetics of Oral Lipid Prodrugs

Kamikihito, a traditional Japanese Kampo medicine, increases the secretion of oxytocin in rats with acute stress

Critical Role of 30 -Downstream Region of pmrB in Polymyxin Resistance in Escherichia coli BL21(DE3)

Extended Graphite Supported Flower-like MnO2 as Bifunctional Materials for Supercapacitors and Glucose Sensing

Comprehensive characterization of Chaenomeles seeds as a potential source of nutritional and biologically active compounds

Exopolysaccharide from Lactobacillus plantarum HY7714 Protects against Skin Aging through Skin-Gut Axis Communication

Modification of the transglucosylation properties of α-glucosidases from Aspergillus oryzae and Aspergillus sojae via a single critical amino acid replacement

Characterisation of ‘Ruby Roman’ Table Grapes (Vitis Labruscana Bailey) by Sensory Evaluation and Analysis of Aroma and Taste Compounds

High-quality sugar production by osgcs1 rice

Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance

Analysis of malto-oligosaccharides and related metabolites in rice endosperm during development

Osmotic Dehydration as a Pretreatment Modulating the Physicochemical and Biological Properties of the Japanese Quince Fruit Dried by the Convective and Vacuum-Microwave Method

Chemical Composition of 21 Cultivars of Sour Cherry (Prunus cerasus) Fruit Cultivated in Poland

Analysis of Transglucosylation Products of Aspergillus niger α-Glucosidase that Catalyzes the Formation of α-1,2- and α-1,3-Linked Oligosaccharides

Comparison of Enzymes Immobilised on Immobeads and Inclusion Bodies: A Case Study of a Trehalose Transferase

High-throughput method to analyze tegafur and 5-fluorouracil in human tears and plasma using hydrophilic interaction liquid chromatography/tandem mass spectrometry

Chemoenzymatic radiosynthesis of 2-deoxy-2-[18F]fluoro-d-trehalose ([18F]-2-FDTre): A PET radioprobe for in vivo tracing of trehalose metabolism

Non-catalytic conversion of chitin into Chromogen I in high-temperature water

Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinase

Conversion of N-acetyl-d-glucosamine to nitrogen-containing chemicals in high-temperature water

Artificial Fusion of mCherry Enhances Trehalose Transferase Solubility and Stability

食品中エリスリトール, マルチトール, ラクチトールおよびトレハロースの HPLC 定量法ならびに LC-MS/MS 確認法

Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are diferentially controlled by cryoprotectants

1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway

Decoration of virus-like particles with an enzymatic activity of biomedical interest

Self-Sustaining Cellulose Nanofiber Hydrogel Produced by Hydrothermal Gelation without Additives

Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition

Effects of Benzyladenine and Light on Post-harvest Calamondin (x Citrofortunella microcarpa) Fruit Color and Quality

Application of a simple methodology to analyze Hydroxypropyl-β-Cyclodextrin in urine using HPLC-LS in early Niemann-Pick disease type C patient

A keratan sulfate disaccharide prevents inflammation and the progression of emphysema in murine models

Effect of mixing different kinds of fruit juice with sour cherry puree on nutritional properties

Peripheral tissue levels and molecular species compositions of N-acyl-phosphatidylethanolamine and its metabolites in mice lacking N-acyl-phosphatidylethanolamine-specific phospholipase D

Quantification of Influenza Neuraminidase Activity by Ultra-Performance Liquid Chromatography and Isotope Dilution Mass Spectrometry

Development and Validation of an Analytical Method for Glucuronolactone in Energy Drinks by Hydrophilic Interaction Liquid Chromatography-electrospray Tandem Mass Spectrometry

Fructose in Breast Milk Is Positively Associated with Infant Body Composition at 6 Months of Age

Evaluation of [14C] and [13C]Sucrose as Blood-Brain Barrier Permeability Markers

The salted radish takuan-zuke shows antihypertension effects in spontaneously hypertensive rats

Nutritional content and health benefits of sun-dried and salt-aged radish (takuan-zuke)

Comparison of nutrient compositions and pharmacological effects of steamed and freeze-dried mature silkworm powders generated by four silkworm varieties

Development, optimization and validation of an HPLC-ELSD method for the analysis of enzymatically generated lactulose and saccharide by-products

Differential fructan accumulation and expression of fructan biosynthesis, invertase and defense genes is induced in Agave tequilana plantlets by sucrose or stress-related elicitors

Nutrition composition differences among steamed and freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons

Biochemical characteristics of Trichoderma atroviride associated with conidium fitness for biological control

Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures

HILIC-MS/MS法によるヒト涙液中tegafurおよび5-fluorouracilの高感度分析法

Evaluation of phytochemicals, antioxidant capacity, and antidiabetic activity of novel smoothies from selected Prunus fruits

N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier

Nutrient compositions of Bombyx mori mature silkworm larval powders suggest their possible health improvement effects in humans

Comparison of catalytic properties of multiple β-glucosidases of Trichoderma reesei

Photosynthesis activates plasma membrane H+-ATPase via sugar accumulation in Arabidopsis leaves

Evaluation of In Vitro Inhibitory Activity of Rye-Buckwheat Ginger Cakes with Rutin on the Formation of Advanced Glycation End-Products (AGEs)

凝集沈殿剤を用いたサッカリンナトリウムおよびアセスルファムカリウムの新規分析法の開発

Comparison of Proximate Components, Free Sugar, Vitamin C and Minerals of 16 Kinds of Honey produced in Korea with Manuka Honey

Antioxidant Activity of Different Grades of Maple Syrup as Determined by the Hydrophilic Oxygen Radical Absorbance Capacity Method

Controlled drug release of silicone-based adhesive-containing cross-linked siloxane powders as a reservoir

Conversion of glucose into 5-hydroxymethylfurfural with boric acid in molten mixtures of choline salts and carboxylic acids

Development of a simple method for quantitation of methanesulfonic acid at low ppm level using hydrophilic interaction chromatography coupled with ESI-MS

Molecular characterization of a new N-acetylneuraminate synthase (NeuB1) from Idiomarina loihiensis

Hesperetin Modifies the Composition of Fecal Microbiota and Increases Cecal Levels of Short-Chain Fatty Acids in Rats

Cloning and starch degradation profile of maltotriose-producing amylases from Streptomyces species

Effects of Soil Application of the Naturally Occurring Form of Abscisic acid (S-ABA) on Development and Quality of Satsuma Mandarin Fruit

The free sugar determination in food by HPLC-NQAD

Applicability of a Forward Osmosis Membrane Process for the Enhancement of Xylose Concentration to Achieve an Effective Biomass Fermentation Process

Rapid and sensitive LC-ESI-MS of gangliosides

First Functional and Mutational Analysis of Group 3 NAcetylneuraminate Lyases from Lactobacillus antri and Lactobacillus sakei 23K

Improved production of 2-keto-3-deoxy-d-glycero-galactononulosonic acid (KDN) using FastPrep-CLEAs

Enantioselectivity in the cytochrome P450-dependent conversion of tegafur to 5-fluorouracil in human liver microsomes

Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes

Non-catalytic synthesis of Chromogen I and III from N-acetyl-D-glucosamine in high-temperature water