PRODUCTS Presto FF-C18
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IMTAKT NON-POROUS ODS COLUMN PRESTO FF-C18

World’s First High-Resolution 2 μm Non-Porous ODS Column

IMTAKT NON-POROUS ODS COLUMN PRESTO FF-C18

FEATURES

Reversed-phase separation of biomolecules and synthetic polymers up to several tens of millions Da

Exceptional separation performance for peptides and proteins

Ultra-high theoretical plate column with 2 μm particle size and 250 mm length

Separation characteristics different from porous ODS columns

Shows high separation performance at low flow rates even with standard HPLC

Demonstrates outstanding performance in reversed-phase separation of proteins such as antibodies

Demonstrates outstanding performance in reversed-phase separation of proteins such as antibodies

Using the non-porous ODS column Presto FF-C18, many peaks previously unseen with traditional wide-pore reversed-phase columns are detected for proteins and monoclonal antibodies (mAb). Peaks that appeared broad with conventional columns are actually multiple components. This is likely due to Presto FF-C18 recognizing conformational differences related to S-S bond positions in protein folding, rather than denaturation. Variations in amino acid sequences, impurities, or glycosylation may also contribute, highlighting the column's ability to detect higher-order protein structures.
The detection of many peaks previously unseen with traditional columns using Presto FF-C18 is expected to advance structural analysis for antibody drugs and other protein therapeutics.

The non-porous ODS column Presto FF-C18 excels in reversed-phase separation not only for proteins but also for biomolecules such as polysaccharides and DNA. It is also applicable to synthetic polymers. Sharp, quantifiable peaks that were difficult to achieve with traditional SEC mode can be obtained. The use of detectors suitable for high polymers, such as ELSD, is expected to introduce a new reversed-phase separation method for polymer analysis.

Low Carryover

Presto FF-C18 exhibits excellent low carryover performance in pharmaceutical LC-MS analysis. This is likely due to its nonporous silica, which avoids non-specific adsorption occurring inside pores.

Reference:
Universal LC-MS Method for Minimized Carryover in a Discovery Bioanalytical Setting
John S. Williams, Stephanie H. Donahue, Hong Gao, Christopher L. Brummel
Bioanalysis (2012) 4(9), 1025-1037
https://www.researchgate.net/publication/225051947_Universal_LC-MS_method_for_minimized_carryover_in_a_discovery_bioanalytical_setting

Key Application

Particularly notable key applications using the Presto FF-C18 column are introduced in this section.

>>>See all Presto FF-C18 Application Database <<<

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