UPDATE: New information has been added to the Applications tab

IMTAKT Intrada Amino Acid Analysis Column for LC-MS

Life Sciences Separation Column

World's First LC-MS Untargeted Amino Acid Analysis Column

FEATURES

Enables direct LC-MS analysis of amino acids without derivatization

Capable of separating isomers like Leu, Ile, and others

Protein constituent amino acids can be measured in 5-10 minutes

Suitable for amino acids, related compounds, dipeptides, etc.

High-throughput analysis in less than 1 minute possible depending on amino acid composition

The conventional methods of amino acid analysis had the following difficulties.

Post-labeling Methods: Utilizes ion-exchange modes, requiring complete separation of amino acids, leading to complex mobile phases and difficulties in shortening analysis time.
Pre-labeling Methods: Allows for short-time analysis using reversed-phase modes, but requires time and equipment for amino acid pre-labeling reactions. Additionally, labeling efficiency is susceptible to sample matrix interference.

Advantages of this Product

Our product allows selective detection of amino acids with different masses using LC-MS. As a pioneering column specialist, we have developed a dedicated stationary phase surface structure, a world-first, to leverage universal LC-MS.

Intrada Amino Acid column, coupled with universal LC-MS, enables rapid analysis without the complexity of labeling. Moreover, with unprecedented column size variations, it achieves high-throughput analysis in as little as 1 minute depending on amino acid compositions.

Its innovative design not only separates Leucine/Isoleucine but also accommodates a wide range of applications for amino acid-related compounds such as β- and γ-amino acid isomers and dipeptides.

Life Sciences Separation Column

FEATURES

Advantages of this Product

[Various Amino Acids]

[Amino Acids and Related Metabolites (Precursors)]

[Amino Acid Isomers]

[Low Molecular Weight Peptides]

>>>See all Intrada Amino Acid Application Database <<<

[Applications for Actual Samples]

Single Quad. MS (LC-MS)

Triple Quad. MS (LC-MS/MS)

TOF/MS

Orbitrap MS

Lysinibacillus piscis sp. nov. isolated from the gut of mottled spinefoot Siganus fuscescens

Changes in TCA cycle and TCA cycle-related metabolites in plasma upon citric acid administration in rats

オホーツクホンヤドカリPagurus ochotensisを食品として利用するための栄養成分分析

Exposure to chronic light-dark phase shifts during the prepartum nonlactating period attenuates circadian rhythms, decreases blood glucose, and increases milk yield in the subsequent lactation

Mass spectrometry-based stable-isotope tracing uncovers metabolic alterations in pyruvate kinase-deficient Aedes aegypti mosquitoes

Control of protein function through oxidation and reduction of persulfidated states

Impact of crop load on nitrogen uptake and reserve mobilisation in Vitis vinifera

Resistance exercise with anti-inflammatory foods attenuates skeletal muscle atrophy induced by chronic inflammation

Disulfide bond formation is not crucial for the heat-induced interaction between β-lactoglobulin and milk fat globule membrane proteins

Glucose-dependent partitioning of arginine to the urea cycle protects β-cells from inflammation

A Variant of SLC1A5 Is a Mitochondrial Glutamine Transporter for Metabolic Reprogramming in Cancer Cells

Effect of half-dry processing on umami components of vegetables and mushrooms

Effects of dietary intake of heat-inactivated Lactobacillus gasseri CP2305 on stress-induced behavioral and molecular changes in a subchronic and mild social defeat stress mouse model

Effects of administration of ascorbic acid and low-dose hydrocortisone after infusion of sublethal doses of lipopolysaccharide to horses

Involvement of NMDA receptors in tremor expression in Aspa/Hcn1 double-knockout rats

Isolation, Characterization and Structure Elucidation of a Novel Lantibiotic From Paenibacillus sp.

Free advanced glycation end product distribution in blood components and the effect of genetic polymorphisms

Comparison of Storage Stability, Volatile Compounds and Sensory Properties between Coarsely-and Finely-Marbled 1+ Grade Hanwoo Beef Loins

Photobiocatalysis by a Lytic Polysaccharide Monooxygenase Using Intermittent Illumination

Carnosine and anserine in chicken can quench toxic acrylamide under cooking conditions: Mass spectrometric studies on adduct formation and characterization

Suppressed Methionine γ-Lyase Expression Causes Hyperaccumulation of S-Methylmethionine in Soybean Seeds

Identifying metabolic features and engineering targets for productivity improvement in CHO cells by integrated transcriptomics and genome-scale metabolic model

Lactobacillus-fermented milk enhances postprandial muscle protein synthesis in Sprague-Dawley rats

Peroxynitrous acid (ONOOH) modifies the structure of anastellin and influences its capacity to polymerize fibronectin

MicroRNA-1291-5p Sensitizes Pancreatic Carcinoma Cells to Arginine Deprivation and Chemotherapy through the Regulation of Arginolysis and Glycolysis

Volatile and non-volatile metabolite changes in 140-day stored vacuum packaged chilled beef and potential shelf life markers

The branched chain aminotransferase IlvE promotes growth, stress resistance and pathogenesis of Listeria monocytogenes

Impacts on product quality attributes of monoclonal antibodies produced in CHO cell bioreactor cultures during intentional mycoplasma contamination events

Effects of Lanthionine and Lysinoalanine on Heat-induced Gelation of Egg White

B38-CAP is a bacteria-derived ACE2-like enzyme that suppresses hypertension and cardiac dysfunction

Tyr-Trp administration facilitates brain norepinephrine metabolism and ameliorates a short-term memory deficit in a mouse model of Alzheimer’s disease

β-Aminoisobutyric Acid Suppresses Atherosclerosis in Apolipoprotein E-Knockout Mice

Comparison of Tastes-Related Components and Eating Quality between Hanwoo Steer and Cow Longissimus thoracis Muscles

Novel method for measuring aquatic bacterial productivity using D10-leucine based on protein synthesis rate

Plasma amino acid levels in a cohort of patients in Turkey with classical phenylketonuria

Kynurenic acid is a potential overlapped biomarker between diagnosis and treatment response for depression from metabolome analysis

Repeated stretch-shortening contraction of the triceps surae attenuates muscle atrophy and liver dysfunction in a rat model of inflammation

An accurate and sensitive analytical method for the simultaneous determination of glycine, methionine and homocysteine in biological matrices by matrix matching strategy and LC-quadrupole-time-of-flight-MS/MS

Integration of Time-Series Transcriptomic Data with Genome-Scale CHO Metabolic Models for mAb Engineering

Bioengineered miR-328-3p modulates GLUT1-mediated glucose uptake and metabolism to exert synergistic antiproliferative effects with chemotherapeutics

Exploration of dipeptidyl-peptidase IV (DPP IV) inhibitors in a low-molecular mass extract of the earthworm Eisenia fetida and identification of the inhibitors as amino acids like methionine, leucine, histidine, and isoleucine

Severity of the autoimmune encephalomyelitis symptoms in mouse model by inhibition of LAT‑1 transporters

Development and application of a multiple reaction monitoring mass spectrometry method for absolute quantification of lysinoalanine and lanthionine in dairy products

Enhancement of amino acid contents by germination in a Korean weedy rice germplasm

Metabolic profiling by reversed-phase/ion-exchange mass spectrometry

Absolute quantitative analysis of intact and oxidized amino acids by LC-MS without prior derivatization

A reliable LC-MS/MS method for targeted analysis of amino acid metabolome: Method validation and application to studies on amino acid dynamics during tumor progression

Plasma amino acid levels in a cohort of patients in Turkey with classical phenylketonuria

Effect of half-dry processing on umami components of vegetables and mushrooms

Amino acids analysis without derivatization by using liquid chromatography quadrupole time-of-flight mass spectrometer (LC-QTOF-MS)

A Paleolithic diet lowers resistant starch intake but does not affect serum trimethylamine-N-oxide concentrations in healthy women

Association Between Serum β-Alanine and Risk of Dementia: The Hisayama Study

The Absence of Pyruvate Kinase Affects Glucose-Dependent Carbon Catabolite Repression in Bacillus subtilis

LC-ESI-MS/MS quantification of carnosine, anserine, and balenine in meat samples

Downregulation of aspartoacylase during the progression of myelin breakdown in the dmy mutant rat with mitochondrial magnesium channel MRS2 defect

Volatile Flavor Components as a Function of Electrical Stimulation and Chiller Aging for m. longissimus and biceps femoris of Hanwoo Beef

Bioinformatic and Functional Evaluation of Actinobacterial Piperazate Metabolism

Impacts of intentional mycoplasma contamination on CHO cell bioreactor cultures

Metabolomics Based on MS in Mice with Diet-Induced Obesity and Type 2 Diabetes Mellitus: the Effect of Vildagliptin, Metformin, and Their Combination

Increase of Arginine Dimethylation Correlates with the Progression and Prognosis of ALS

Consumer Perception of Snack Sausages Enriched with Umami-Tasting Meat Protein Hydrolysates

Determination of Glycine in Body Fluids at Trace Levels Using the Combination of Quadrupole Isotope Dilution Strategy and Liquid Chromatography-Quadrupole Time of Flight-Tandem Mass Spectrometry

Use of mTRAQ Derivatization Reagents on Tissues for Imaging Neurotransmitters by MALDI Imaging Mass Spectrometry: the Triple Spray Method

Growth and Physiological Characteristics in a Halophyte Suaeda glauca under Different NaCl Concentrations

Dosing Time-Dependent Changes in the Anti-tumor Effect of xCT Inhibitor Erastin in Human Breast Cancer Xenograft Mice

Real-time Quantification and Supplementation of Bioreactor Amino Acids to Prolong Culture Time and Maintain Antibody Product Quality

Analytical Strategies for the Determination of Amino Acids: Past, Present and Future Trends