User Reports
SCIEX
U.S.A.
Ultra-High Sensitivity LC-MS Analysis of Testosterone
Non-ionic steroid hormones often have poor ionization efficiency, resulting in low MS sensitivity.
By derivatizing testosterone with a novel quaternary aminooxy reagent and analyzing it using a Sciex QTRAP 5500 mass spectrometer and a Cadenza CL-C18 column, an LLOQ of below 1 pg/mL for standard samples and below 50 pg/mL for DBS samples was achieved.
The QTRAP 5500 mass spectrometer and the Cadenza CL-C18 column are effective for high-sensitivity analysis of derivatized steroid hormones.
Rapid Metabolite Identification Using MetabolitePilot Software and TripleTOF 5600 System
MetabolitePilot software by Sciex enables comprehensive metabolite identification in the field of drug discovery.
By incubating bromocriptine with rat liver microsomes and analyzing its metabolites using the TripleTOF 5600 system and the Cadenza CD-C18 column for LC-MS/MS analysis, each metabolite could be identified from the MS spectra and UV absorption information of each peak using the software.
The combination of MetabolitePilot software and the TripleTOF 5600 mass spectrometer, along with the Cadenza CD-C18 column, is effective in metabolomics studies.
Identification of Reactive Metabolites Using Fast Scanning Linear Ion Trap
Analyzing reactive metabolites (epoxides, quinones, free radicals, reactive oxygen species, etc.) formed during drug metabolism is crucial for assessing the safety of candidate compounds. Trapping assays for reactive metabolites typically use trapping agents such as glutathione (GSH), its ethyl ester (GSH-EE), and N-acetylcysteine (NAC).
Using Sciex's QTRAP 5500 and the Cadenza CD-C18 column, we performed LC-MS/MS analysis of troglitazone metabolites conjugated with various trapping agents. The results showed that high-sensitivity and rapid measurement is possible even at a reaction concentration of 1 μM.
The QTRAP 5500 mass spectrometer and the Cadenza CD-C18 column are expected to play a significant role in measuring reactive metabolites.