Analysis of Sugars and Erythritol in Energy/Vitamin Supplement Beverages Using LC-ELSD

ELSD (Evaporative Light Scattering Detection) is effective for analyzing sugars that are difficult to detect with UV. Additionally, silica-based amino columns required for normal-phase separation of sugars need to address column bleed due to the self-hydrolysis of amino groups.

Using the PerkinElmer Flexar/Chromera LC-ELSD system and the low-bleed Unison UK-Amino column, good linearity in the range of tens to hundreds of ppm was achieved for erythritol, arabinose, xylose, fructose, mannose, glucose, sucrose, and maltose. The method was also confirmed to be applicable to commercially available beverages.

The combination of the PerkinElmer LC-ELSD system and the Unison UK-Amino column is effective for sugar analysis.

• APPLICATION NOTE

Analysis of Non-Derivatized Amino Acids Using LC-MS

To address concerns with traditional derivatization methods, such as side reactions, the effects of derivatization reagents, and sample degradation, a straightforward and reproducible non-derivatized LC-MS method has been gaining attention.

Using the PerkinElmer Altus™ HPLC System with the Altus SQ MS detector and the Intrada Amino Acid column, specifically designed for LC-MS analysis of amino acids, we investigated 17 different amino acid standard solutions used for protein composition analysis. The results demonstrated that, except for Ala and Cys, amino acids exhibited linearity in the range of 6 - 100 nmol/mL with a correlation coefficient of 0.999. Additionally, histidine (His) at a concentration of 100 nmol/mL showed remarkably high reproducibility with a 0.016% RSD across 10 injections. The limit of detection (LOD) was generally below 1 nmol/mL for all amino acids, except Ala, indicating very high sensitivity for a Single MS setup.

The combination of the PerkinElmer Altus SQ MS detector and Intrada Amino Acid column allows for convenient and rapid non-derivatized amino acid analysis.

• APPLICATION NOTE