User Reports
Mallinckrodt
U.S.A.
Isopropanol Mobile Phase Beneficial for Reversed-phase Analysis of Peptide Pharmaceuticals
In gradient separation of peptides detected at a wavelength of 220 nm, the commonly used acetonitrile can cause baseline noise derived from the solvent, which interferes with the analysis.
The authors found that using isopropanol (IPA) instead of acetonitrile yields chromatograms with less baseline noise.
Cadenza CD-C18 is a low-pressure, high-theoretical-plate column, and when combined with IPA, it allows for the tracking of peptide oxidation processes.
Additionally, the wide-pore reversed-phase column Intrada WP-RP was found to provide excellent separation for large peptides.
Cadenza CD-C18 and Intrada WP-RP are effectively utilized in peptide analysis.