User Reports
Biotage
U.S.A.
LC-MS/MS Analysis of Vitamin B3 and Metabolites using High-Throughput Solid-Phase Extraction
They used Biotage EVOLUTE SCX-3 for the pretreatment of human serum and conducted LC-MS/MS analysis of Vitamin B3 (Niacin) and its metabolites—nicotinic acid, nicotinamide, and nicotinuric acid.
As a result, a sensitivity of 20 ng/mL (MS Q3) was achieved.
It was found that the combination of EVOLUTE SCX-3 and Scherzo SM-C18 is optimal for the analysis of Vitamin B3 and its metabolites in serum.
LC-MS/MS Analysis of Biotin using High-Throughput Solid-Phase Extraction
They conducted LC-MS/MS analysis of biotin (Vitamin B7) using Biotage's EVOLUTE AX for pretreating human serum.
As a result, they achieved an MS (Q3) sensitivity of 6 ng/mL.
It was found that the combination of EVOLUTE AX and Scherzo SM-C18 is optimal for the analysis of biotin in serum.
- Imtakt Technical Information, TI749E
(Courtesy of Frank Kero, Biotage, USA)