User Reports
Inha University
Korea
Rapid Quantitation Method for Antiepileptic Drugs (AEDs) in Plasma by LC-MS/MS
In the field of clinical pharmacokinetics (TDM), drug analysis in plasma using LC-MS is essential. However, matrix effects from plasma proteins can cause ion suppression in MS, compromising quantification. Typically, plasma samples require pre-treatment (deproteinization), which is time-consuming and incurs running costs.
Cadenza HS-C18 uses an ODS packing material that can be dispersed in water and has the feature of not adsorbing plasma proteins. The authors demonstrated that this characteristic allows for a direct injection LC-MS/MS analysis method by simply diluting and centrifuging plasma for the following antiepileptic drugs:
Gabapentin, Levetiracetam, Tolbutamide, Oxcarbazepine, Carbamazepine, Sulfamethoxazole, Primidone, Topiramate, Zonisamide, Phenytoin, Lamotrigine
- Plasma Sample Preparation Method -
- Dilute 10 μL of plasma with 500 μL of 2 mM ammonium acetate
- Mix for 15 seconds using a vortex mixer
- Centrifuge at 13,000 rpm for 5 minutes
- Inject 1 μL of the supernatant into Cadenza HS-C18
Cadenza HS-C18 is a highly convenient column for direct plasma injection LC-MS quantification, requiring only plasma dilution and centrifugation.
- A simple and reliable method for simultaneous quantification of antiepileptic drugs in plasma using LC-MS/MS combined with direct injection column (Poster at ASMS)
(Courtesy of Dr. Kwang-Youl Kim, Inha University, KOREA)
Reference:
Direct injection LC-MS/MS method for the determination ofteicoplanin in human plasma
Kwang-Youl Kim, Sang-Heon Cho, Yong-Hyun Song, Moon-Suk Nam, Cheol-Woo Kim (Inha University, KOREA)
Journal of Chromatography B, 1008 (2016) 125-131
LC-MS/MS Analysis of Non-Opioid Drugs in Human Plasma
In the field of emergency medicine, it is crucial to identify and quantify drugs in plasma as quickly as possible. The authors developed a method for simultaneous LC-MS/MS analysis of the following 12 important drugs in about 5 minutes using the versatile C8 column Unison UK-C8:
Acetaminophen, Amitriptyline, Chlorpromazine, Cimetidine, Diazepam, Diphenhydramine, Ephedrine, Imipramine, Metoclopramide, Propranolol, Tramadol, Zolpidem
The Unison UK-C8 column is effective for LC-MS analysis of non-opioid drugs, including strongly basic compounds.
- Imtakt Techical Information, TI697E
(Courtesy of Dr. Kwang-Youl Kim, Inha University, KOREA)
Reference:
Application of liquid chromatography tandem mass spectrometry for the simultaneous quantification of multiple non-opioid drugs in human plasma
Kwang-Youl Kim, Ju-Hee Kang, Cheol-Woo Kim and Moonsuk Nam (Inha Univ., Korea)
Mol Cell Toxicol (2011) 7: 185-189