Group Separation of Rice Bran Oil Compounds

Rice bran oil (rice oil) contains various groups of compounds with different structures as follows. These groups consist of subtly different molecular species:

TAG: Triglycerides
STsFFA: Sterol Fatty Acid Esters
OZ: Orizanol compounds
FFA: Fatty acids

With a conventional ODS column, numerous molecular species peaks are generated to identify and retain fine solute structures, making it challenging to separate compounds into distinct groups with similar properties. The Unison UK-C1 column, lacking a long alkyl chain length, does not exhibit high hydrophobic identification capability. However, while it may be difficult to identify individual molecular species, it enables separation on a group-by-group basis for compounds with similar properties.

This application demonstrates that it is possible to separate these four lipid groups on a group-by-group basis. The Unison The UK-C1 column is expected to be an effective method for measuring the content of each group separately.

• Group Separation of Compounds in Rice Bran Oil
  

  (Courtesy of Hashimoto)

Comprehensive Analysis of Functional Components in Rice Bran Oil

Rice bran oil (rice oil) is rich in numerous valuable components, including tocopherols, tocotrienols, plant sterols, triterpene alcohols, and γ-oryzanol, which is an ester of these with ferulic acid. These functional components are being actively applied in pharmaceuticals and supplements.

We investigated a comprehensive analytical method for these highly hydrophobic compounds using the high-resolution Cadenza CD-C18 column and LC-MS. As a result, isocratic analysis of 14 components was achieved using APCI ionization.

The 250 mm long high-resolution Cadenza CD-C18 column demonstrated excellent performance in separating highly hydrophobic compounds.

• Comprehensive LC-MS Analysis of Functional Components in Rice Bran Oil
  

  (Courtesy of Hashimoto and Sawada)