Development of Sphingolipid Measurement System by LC-IT-TOF MS

Sphingolipids, which share a common ceramide structure, are highly hydrophobic compounds based on two types of long-chain alkyl groups. For these compounds, a wide-pore ODS column with a pore size of 30 nm, such as Cadenza CW-C18, provides better retention and separation balance compared to standard low-molecular ODS columns with typical pore sizes (10-13nm).

The authors developed a measurement system for intracellular ceramides using a wide-pore ODS column and LC-IT-TOF MS, achieving sufficient sensitivity and high throughput. This method is considered useful for the analysis of ceramides as biomarkers for Alzheimer's disease (AD).

The wide-pore ODS column Cadenza CW-C18 is effective for the LC-MS analysis of highly hydrophobic sphingolipids.

• Oral Presentation Data

  (Provided by Professor Mitsuhiro Nakamura, Department of Practical Pharmacy, Gifu Pharmaceutical University)

Reference:
Interferon-γ Decreases Ceramides with Long-Chain Fatty Acids: Possible Involvement in Atopic Dermatitis and Psoriasis.
Tawada C, Kanoh H, Nakamura M, Fujisawa T, Banno Y, Seishima M.
J Invest Dermatol 2014 ; 134 : 712-718. doi: 10.1038/jid.2013.364.

Development of a Novel Micafungin Blood Concentration Measurement Method Using a Hydrophobic/Hydrophilic Hybrid ODS Column for Direct Plasma Injection

Online drug analysis methods in plasma typically require a deproteinization pretreatment column in addition to the analytical column, complicating the system. Cadenza HS-C18 is a hydrophobic/hydrophilic hybrid ODS column with a hydrophilic surface structure that can exclude plasma proteins, making it suitable for simple gradient LC or LC-MS systems without the need for deproteinization steps.

The authors developed a plasma analysis method for micafungin, an echinocandin antifungal drug, using Cadenza HS-C18 and HPLC-fluorescence detection method, employing an analogue as an internal standard. They obtained favorable results in terms of linearity, recovery rate, and intra-/inter-day variability. This method is considered effective for the rapid diagnosis of fungal infections in emergency and intensive care settings.

The serum (plasma) direct injection column Cadenza HS-C18 is also effective in the field of therapeutic drug monitoring (TDM).

• Oral Presentation Data

  (Provided by Professor Mitsuhiro Nakamura, Department of Practical Pharmacy, Gifu Pharmaceutical University)

Reference:
Direct-injection HPLC method of measuring micafungin in human plasma using a novel hydrophobic/hydrophilic hybrid ODS column.
Uranishi H, Nakamura M, Nakamura H, Ikeda Y, Otsuka M, Kato Z, Tsuchiya T.
J Chromatogr B 2011 ; 879 ; 1029-1032. doi: 10.1016/j.jchromb.2011.03.008.

Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and AKT activation.
Nemoto S, Nakamura M, Osawa Y, Kono S, Itoh Y, Okano Y, Murate T, Hara A, Ueda H, Nozawa Y, Banno Y.
J Biol Chem 2009 ; 284 : 10422-10432. doi: 10.1074/jbc.M900735200.

Implications of sphingosine kinase 1 expression level for the cellular sphingolipid rheostat: relevance as a marker for daunorubicin sensitivity of leukemia cells.
Sobue S, Nemoto S, Murakami M, Ito H, Kimura A, Gao S, Furuhata A, Takagi A, Kojima T, Nakamura M, Ito Y, Suzuki M, Banno Y, Nozawa Y, Murate T.
Int J Hematol 2008 ; 87 : 266-275. doi: 10.1007/s12185-008-0052-0.