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SEDERE S.A.S.
France

Scherzo SM-C18

Simultaneous Analysis of Water-Soluble and Fat-Soluble Vitamins Using ELSD

One of the challenging tasks in analytical chemistry is the simultaneous analysis of water-soluble and fat-soluble vitamins. Furthermore, due to weak UV absorption of some vitamins, simultaneous analysis with UV detection is considered quite difficult.

The authors reported simultaneous analysis of the following vitamins using highly sensitive ELSD and a dual ion-exchange multi-mode ODS Scherzo SM-C18:

Vitamin B1, B8, B6, C, B5, B9, B12, B2, B7, A, K2, D2, D3, E, K1

Scherzo SM-C18

Simultaneous Analysis of Organic Acids and Phenolic Acids Using ELSD

Organic acids present a challenging task for analysis due to their weak UV absorption and high polarity, making them difficult to analyze using standard ODS columns.

The authors reported simultaneous analysis of the following organic acids using highly sensitive ELSD and a dual ion-exchange multi-mode ODS Scherzo SM-C18:

Quinic acid, malic acid, tartaric acid, succinic acid, citric acid, gallic acid, lactic acid, protocatechuic acid, 4-hydroxyphenylacetic acid, chlorogenic acid, syringic acid, gentisic acid

Unison UK-Amino

Simultaneous Analysis of Polyols, Monosaccharides, Disaccharides, and Oligosaccharides Using ELSD

Sugars are challenging to analyze with ODS columns due to their weak UV absorption and high polarity. Typically, normal-phase separation using amino columns is employed. The traditional detection method is RI (refractive index detection); however, it is sensitive to baseline changes caused by the mobile phase, making gradient elution impossible. Additionally, the sensitivity is not very high.

ELSD allows gradient elution using a volatile mobile phase and offers higher sensitivity compared to RI detection.

The authors reported simultaneous analysis of the following sugars using highly sensitive ELSD and a low-bleed amino column Unison UK-Amino:

Glycerol, rhamnose, erythritol, arabinose, xylose, fructose, sorbitol, mannose, galactose, glucose (dextrose), inositol, sucrose (table sugar), maltulose, lactose (milk sugar), maltose, trehalose, raffinose, maltotriose, nystose, maltotetraose