Column Comparison for Metabolite Profiling

In mass spectrometry-based metabolomics analysis, many endogenous metabolites are highly polar, making it difficult to retain them on conventional ODS columns.

The authors compared columns for 31 metabolomics-related compounds and demonstrated that the dual ion-exchange multi-mode ODS column Scherzo SM-C18 could retain and analyze more compounds than other columns.

Scherzo SM-C18 proves to be effective in metabolomics analysis.

• Imtakt Techical Information, TI652E

  (Courtesy of Oscar Yanes, Ph.D.,
  Metabolomics Platform of the Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders[CIBERDEM],
  University Rovira i Virgili, SPAIN)

Reference:
Expanding Coverage of the Metabolome for Global Metabolite Profiling
Oscar Yanes, Ralf Tautenhahn, Gary J. Patti, and Gary Siuzdak
Analytical Chemistry, 2011, 83(6), pp 2152-2161