UPDATE: New information has been added to the Applications tab

Acid Resistance and Wide-Polarity Separation, High-Performance C8 Column

FEATURES

World's highest theoretical plate number column: 50,000 plates (250x4.6mm)

Wide selectivity from 100% aqueous to non-aqueous mobile phases

Unique end-capping: basic, acid-resistant, alkali-resistant

Wide range of column sizes: inner diameter 0.075 - 28mm / length 10-500mm

High-pressure resistance options
(HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa)

MAIN SPECIFICATIONS

Particle size:	3μm (Unison UK-C8) 5μm (Unison US-C8)
Base material:	Fully porous high-purity silica gel
Pore size:	13nm
Ligand:	Octadecyl group
End-capping:	Present (polymeric end-capping)
Target molecular weight:	Below 10kDa
pH range:	pH 1.5-9
Maximum pressure:	250bar / 3,500psi / 25MPa HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa
USP Code:	L7

Unison UK-C8 is a versatile column that offers slightly shorter retention compared to ODS columns but excels in separation balance.
It features excellent acid and alkali resistance due to its unique “polymeric end-capping,” promising a long lifespan comparable to conventional ODS columns.

Advantages of this product

Performance count, comparable to ODS columns
Superior end-capping efficiency, potentially improving peak shape for basic compounds compared to ODS columns
Improved separation of highly polar substances with 100% aqueous mobile phases
Enhanced separation of isomers and related compounds
Excellent response for hydrophobic substances
Suitable for drug analysis using LC-MS

Unison UK-C8 offers performance that challenges the conventional notion that “reversed-phase separation equals ODS columns.”

Low Carryover [Optimal for LC-MS]

Unison UK-C8 demonstrates excellent low carryover performance in the LC-MS analysis of pharmaceuticals. This is attributed to a unique polymeric end-capping technique and a method of development that differs from conventional filler synthesis.

Reference:
Universal LC-MS Method for Minimized Carryover in a Discovery Bioanalytical Setting
John S. Williams, Stephanie H. Donahue, Hong Gao, Christopher L. Brummel
Bioanalysis (2012) 4(9), 1025-1037
https://www.researchgate.net/publication/225051947_Universal_LC-MS_method_for_minimized_carryover_in_a_discovery_bioanalytical_setting

FEATURES

MAIN SPECIFICATIONS

Advantages of this product

Low Carryover [Optimal for LC-MS]

[Pharmaceuticals and Agrochemicals]

[Agrochemicals]

[Food and Biochemistry]

[Other]

>>>See all Unison UK-C8 Application Database <<<

IONICS Mass Spectrometry (PerkinElmer)

Neuritogenic steroid glycosides from crown-of-thorns starfish: Possible involvement of p38 mitogen-activated protein kinase and attenuation of cognitive impairment in senescence-accelerated mice (SAMP8) by peripheral administration

A Single‑ and Multiple‑Dose Pharmacokinetic Study of Oral Perampanel in Healthy Chinese Subjects

Analytical Method for Determination of Laccaic Acids in Foods with HPLC-PDA and Monitoring

In Vivo Delivery of Therapeutic Molecules by Transplantation of Genome-Edited Induced Pluripotent Stem Cells

Nonclinical safety evaluation of food colorant lac dye via systematic toxicity profiling with assessment of in vivo antigenic potential

Modeling of Anticancer Sulfonamide Derivatives Lipophilicity by Chemometric and Quantitative Structure-Retention Relationships Approaches

Intravenous Perampanel as an Interchangeable Alternative to Oral Perampanel: A Randomized, Crossover, Phase I Pharmacokinetic and Safety Study

Development of LC-MS/MS analytical methods for metalaxyl in Atractylodes macrocephala Koidzumi and Achyranthes japonica Nakai

Comparative urinary globotriaosylceramide analysis by thin-layer chromatography-immunostaining and liquid chromatography-tandem mass spectrometry in patients with Fabry disease

Population Pharmacokinetic Analysis and Dosing Optimization Based on Unbound Daptomycin Concentration and Cystatin C in Nonobese Elderly Patients with Hypoalbuminemia and Chronic Kidney Disease

Changes in Adrenal Androgens and Steroidogenic Enzyme Activities From Ages 2, 4, to 6 Years: A Prospective Cohort Study

Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats

Predictability of human pharmacokinetics of diisononyl phthalate (DINP) using chimeric mice with humanized liver

Rapid Identification of Anthocyanin in Ficus Aurata Fruits by Liquid Chromatography-Mass Spectrometry Approach

Influence of Hydrolysis Susceptibility and Hydrophobicity of SN-38 Nano-Prodrugs on Their Anticancer Activity

Simultaneous Assay of Fecal Short-Chain Fatty and Bile Acids and Ratio of Total Bile Acids to Butyrate in Colon Cancer

Measurement of serum steroid profiles by HPLC-tandem mass spectrometry

Human urinary concentrations of monoisononyl phthalate estimated using physiologically based pharmacokinetic modeling and experimental pharmacokinetics in humanized-liver mice orally administered with diisononyl phthalate

Practical one-step glucuronidation via biotransformation

Lycosides, unusual carotenoid-derived terpenoid glycosides from a vegetable juice, inhibit asexual reproduction of the plant pathogen Phytophthora

Local dermal delivery of cyclosporin A, a hydrophobic and high molecular weight drug, using dissolving microneedles

Cholesterol Unbound RORγt Protein Enables a Sensitive Inverse Agonist Screening

Assessment of multiple cytochrome P450 activities in metabolically inactivated human liver microsomes and roles of P450 2C isoforms in reaction phenotyping studies

2-S-cysteinylhydroquinone is an intermediate for the firefly luciferin biosynthesis that occurs in the pupal stage of the Japanese firefly, Luciola lateralis

Identification of a freshness marker metabolite in stored soybean sprouts by comprehensive mass-spectrometric analysis of carbonyl compounds

自動車塗装廃水からのメラミン分解菌の取得とメラミン分解方法の基礎的研究

Pharmacokinetic and pharmacodynamic evaluation according to absorption differences in three formulations of ibuprofen

Universality of the Phytophthora mating hormones and diversity of their production profile

Simultaneous Analysis of Oil-Soluble, Basic, and Acidic Illegal Dyes in Foods Using Liquid Chromatography?Diode-Array Detection

Determination of 3-hydroxypropylmercapturic acid in urine by three column-switching high-performance liquid chromatography with electrochemical detection using a diamond electrode

An inter-laboratory cross-validation study for the determination of perampanel in human plasma by liquid chromatography assays

Pathogenic microorganisms, heavy metals, and antibiotic residues in seven Korean freshwater aquaculture species

Drug Release is Determined by the Chain Length of Fatty Acid-Conjugated Anticancer Agent as One Component of Nano-Prodrug

In Vitro Metabolic Pathways of the New Anti-Diabetic Drug Evogliptin in Human Liver Preparations

Organic anion transporter 3- and organic anion transporting polypeptides 1B1- and 1B3-mediated transport of catalposide

Synthesis and Characterization of a New Hydroquinone Derivative: Disodium p-Phenylene Diisostearyl Diphosphate

Synthesis of deleobuvir, a potent hepatitis C virus polymerase inhibitor, and its major metabolites labeled with carbon-13 and carbon-14

Quantification of serum C-peptide by isotope-dilution liquid chromatography-tandem mass spectrometry: Enhanced detection using chemical modification and immunoaffinity purification

Induction of a Hardening Phenomenon and Quantitative Changes of Ceramides in Stratum Corneum

Indirect Quantitation of Saxitoxin by HPLC with Post-column Oxidation and Fluorometric Detection

Exposure evaluation of adult male Japanese smokers switched to a heated cigarette in a controlled clinical setting

Biosynthesis of Firefly Luciferin in Adult Lantern: Decarboxylation of L-Cysteine Is a Key Step for Benzothiazole Ring Formation in Firefly Luciferin Synthesis

Staphylococcus aureus Penicillin-Binding Protein 2 Can Use Depsi-Lipid II Derived from Vancomycin-Resistant Strains for Cell Wall Synthesis

Single-Dose Pharmacokinetic Properties, Bioavailability, and Tolerability of Two Lamivudine 100-mg Tablet Formulations: A Randomized Crossover Study in Healthy Chinese Male Subjects

The Blood-Brain Barrier Permeability of 18β-Glycyrrhetinic Acid, a Major Metabolite of Glycyrrhizin in Glycyrrhiza Root, a Constituent of the Traditional Japanese Medicine Yokukansan

Correlations between Chemical Compositions and Retention Times of Methacrylate Random Copolymers Using LC-ESI-MS

Universal LC-MS method for minimized carryover in a discovery bioanalytical setting

Application of liquid chromatography tandem mass spectrometry for the simultaneous quantification of multiple non-opioid drugs in human plasma

Increased Expression of Hepatic Organic Cation Transporter 1 and Hepatic Distribution of Metformin in High-fat Diet-induced Obese Mice

Long-Chain N-Vanillyl-acylamides from Capsicum Oleoresin

Integration of photocatalysis and membrane distillation for removal of mono- and poly-azo dyes from water

Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis -A review

Evaluation of the antioxidant activity of the fruiting body of Phellinus linteus using the on-line HPLC-DPPH method

Effect of process parameters on photodegradation of Acid Yellow 36 in a hybrid photocatalysis-membrane distillation system

Effect of Oral Ketoconazole on Oral and Intravenous Pharmacokinetics of Simvastatin and Its Acid in Cynomolgus Monkeys

Effectiveness of photodecomposition of an azo dye on a novel anatase-phase TiO2 and two commercial photocatalysts in a photocatalytic membrane reactor (PMR)

Isoflavone aglycones production from isoflavone glycosides by display of β-glucosidase from Aspergillus oryzae on yeast cell surface

Rate-Limiting Steps of Oral Absorption for Poorly Water-Soluble Drugs in Dogs; Prediction from a Miniscale Dissolution Test and a Physiologically-Based Computer Simulation

Determination of testosterone concentrations in rat plasma using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry combined with ethyl oxime and acetyl ester derivatization

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