Complementing ODS Column Analysis
Exceptional Water-Resistant C1 Stationary Phase
FEATURES
New C1 stationary phase that resists hydrolysis in acidic mobile phases such as formic acid or TFA
Broad applicability for a wide range of compounds from high to low polarity
Analysis of lipophilic compounds that are difficult to elute with ODS columns
Different separation selectivity compared to ODS
Convenient Performance 3μm particle columns
High-pressure resistance options
(HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa)
MAIN SPECIFICATIONS
| Particle size: | 3μm (Unison UK-C1) 5μm (Unison US-C1) |
| Base material: | Fully porous high-purity silica gel |
| Pore size: | 13nm |
| Ligand: | Methyl Group |
| Target molecular weight: | Below 10kDa |
| pH range: | pH 1.5-9 |
| Maximum pressure: | 250bar / 3,500psi / 25MPa HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa |
| USP Code: | L13 |
Traditional C1 columns have faced challenges in durability, particularly acid resistance, when used with aqueous mobile phases.
In developing the Unison UK-C1 column, we applied innovative stationary phase design technologies cultivated through the creation of unique products such as the Intrada and Scherzo columns.
As a result, we successfully achieved a C1 surface structure with high resistance to hydrolysis.
While the C1 stationary phase is less hydrophobic compared to ODS, the strong influence of the polar groups on the silica surface may result in selectivity different from that of ODS columns.
The Unison UK-C1 column offers potential for analyses using general-purpose mobile phases, enabling the separation of impurity peaks undetectable with conventional ODS columns. Additionally, it facilitates the elution of highly hydrophobic compounds, such as long-chain alkyl compounds, which are challenging for ODS columns.