PRODUCTS Nardis Columns NARDIS ND-NX
UPDATE: New information has been added to the applications area
IMTAKT HPLC Column Nardis ND-NX

Normal-phase + dual Ion exchange

When conventional columns struggle with retention and separation,

IMTAKT HPLC Column Nardis ND-NX

MAIN SPECIFICATIONS

Particle size: 3μm
Pore size: 13nm
Stationary phase: Weak anion group + Weak cation group
Separation mode: Normal-phase + Both ion-exchange
Target molecular weight: Below 10kDa
pH range of use: 1.5 - 8
Column pressure resistance: 3600psi / 250bar / 25MPa (1-6mm ID)

CAUTION

The dual ion-exchange column is not designed for simultaneous analysis of cationic and anionic substances. The stationary phase contains ion ligands, so it is essential to use a pH modifier and appropriate ion strength in the mobile phase.

As it operates in ion-exchange mode, "gradient elution" is necessary when using this column.

Since the stationary phase consists of weak ion-exchange ligands, substances with multiple ionic functional groups or weakly ionizable substances with a pKa of 4-5 may result in multiple peaks under certain analysis conditions.

Please note that this product does not guarantee retention or peak shape for all substances. Optimization of the analysis requires careful adjustment of the mobile phase (organic solvent, pH, ion strength).

Dual Ion-Exchange Capability

Nardis ND-NX (normal-phase) is equipped with weak anion and weak cation groups. This improves the retention and separation of strongly ionic compounds.

Characteristics of Normal-phase + Dual Ion-Exchange Mode Nardis ND-NX

Nardis ND-NX is a "normal-phase + dual ion-exchange" column that incorporates weak anion and weak cation groups.

In normal-phase mode, solutes are retained when the polarity of the mobile phase is low and eluted when the polarity is increased.

Acidic compounds are strongly retained at acidic pH and eluted as the pH and ion strength are increased.

Basic compounds are strongly retained at neutral pH and eluted as the pH is lowered and ion strength is increased.

Separation Improvement of Amino Column (Unison UK-Amino)

This column enhances the retention and separation of nucleic acid-related compounds that are highly polar and not strongly ionizable.

This column also improves the retention and separation of basic substances.

This column has the potential to improve monosaccharide retention.

This column has the potential for simultaneous analysis of uronic acids and amino sugars.

This column enables group separation of pentoses, hexoses, uronic acids, and amino sugars.

Alternative to Silica Column (Unison UK-Silica)

This column can serve as an alternative to traditional silica for aqueous normal-phase mode.

Application to Phosphate Compounds (Metal-Free Column)

Using the metal-free version of this column, applying normal-phase + ion-exchange mode allows for easy retention and separation of phosphate compounds such as nucleotides and coenzymes.

Applications of Nardis ND-NX

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