Reversed-phase + dual ion-exchange
When conventional columns struggle with retention and separation,
MAIN SPECIFICATIONS
| Particle size: | 3μm |
| Pore size: | 13nm |
| Stationary phase: | Phenyl group + Weak anion group + Weak cation group |
| Separation mode: | Reversed-phase + Both ion-exchange |
| Target molecular weight: | Below 10kDa |
| pH range of use: | 1.5 - 8 |
| Column pressure resistance: | 3600psi / 250bar / 25MPa (1-6mm ID) |
CAUTION
The dual ion-exchange column is not designed for simultaneous analysis of cationic and anionic substances. The stationary phase contains ion ligands, so it is essential to use a pH modifier and appropriate ion strength in the mobile phase.
As it operates in ion-exchange mode, "gradient elution" is necessary when using this column.
Since the stationary phase consists of weak ion-exchange ligands, substances with multiple ionic functional groups or weakly ionizable substances with a pKa of 4-5 may result in multiple peaks under certain analysis conditions.
Please note that this product does not guarantee retention or peak shape for all substances. Optimization of the analysis requires careful adjustment of the mobile phase (organic solvent, pH, ion strength).
Dual Ion-Exchange Capability
Nardis ND-RX (reversed-phase system) features phenyl groups (reversed-phase) as well as weak anion and weak cation groups. This enhances the separation of mainly non-ionic and strongly ionic compounds.
Characteristics of Nardis ND-RX in Reversed-Phase and Dual Ion-Exchange Modes
Nardis ND-RX is a "reversed-phase + both ion-exchange" column with phenyl, weak anion, and weak cation groups.
In reversed-phase mode, solutes are adsorbed when the polarity of the mobile phase is high and elute when the polarity is lowered (increasing hydrophobicity).
Acidic compounds are strongly retained at acidic pH and elute when the pH and ionic strength are increased.
Basic compounds are strongly retained at neutral pH and elute when the pH is lowered and the ionic strength is increased.
Improved Separation compared to Phenyl Columns (Unison UK-Phenyl)
When separation is difficult with a Phenyl stationary phase, such as with steroids and their derivatives, electrostatic interactions with both ion ligands may enable separation. It may also serve as an alternative to biphenyl columns.
- Androstenedione Biosynthesis Pathway
Increased acetonitrile under acidic conditions
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Steroid HormonesEthinylestradiol, Estrone
Increase in Acetonitrile under Acidic Acetic Conditions
This column differs from the phenyl stationary phase in the retention and separation of highly polar substances.
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Adenosine Metabolites
Adenosine, Inosine, Hypoxanthine
Increased acetonitrile in the presence of ammonium formate
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Heterocyclic Aromatic Compounds
Pyrazole, Pyrimidine, Imidazole
Increased acetonitrile in the presence of ammonium formate
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Indole Derivatives
Indole, Oxindole, Isatin
Increased acetonitrile in the presence of ammonium formate
This column is also effective in improving the retention and separation of acidic and basic substances.
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Benzenesulfonic Acid-Related Compounds
Gradient of pH and ionic strength from formic acid to ammonium formate under reversed-phase conditions
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Food Preservatives
Sorbic Acid, Benzoic Acid
Increased acetonitrile under acidic conditions with formic acid
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Catecholamines
Indole, Oxindole, Isatin
Gradient of pH and ionic strength from ammonium acetate to acetate buffer under reversed-phase conditions
Different Selectivity Compared to Multi-Mode ODS Column (Scherzo C18)
This column offers different selectivity compared to the "ODS + dual ion-exchange" multi-mode reversed-phase column, Scherzo C18.
- Anticancer Drug, Mercaptopurine
Increase in acetonitrile under acidic conditions
- Hydroxybenzenesulfonic Acid
pH and ion strength gradient from acidic to neutral conditions