Polar End-Capping - General-Purpose ODS Column
FEATURES
Balanced universal ODS stationary phase for compounds ranging from high to low polarity
Excellent Response for Basic Compounds in Formic Acid Mobile Phase
Superior stereoselectivity and separation performance of isomers
User-friendly high-theoretical-plate columns with 3 μm (CX-C18) and 5 μm (5CX-C18) particles
Wide Range of Column Sizes: Inner Diameter 0.075 - 28mm / Length 10 - 500mm
MAIN SPECIFICATIONS
| Particle size: | 3μm (Cadenza CX-C18) 5μm (Cadenza 5CX-C18) |
| Base material: | Fully Porous High-Purity Silica Gel |
| Pore size: | 12nm |
| Ligand: | Octadecyl group |
| End-capping: | Polar Group End-Capping |
| Target molecular weight: | Below 10kDa |
| pH range: | pH 2-10 |
| Maximum pressure: | 250bar / 3,500psi / 25MPa HP: 500bar / 7,500psi / 50MPa, UP: 1,000bar / 15,000psi / 100MPa |
| USP Code: | L1 |
Traditional silica-based ODS columns containing hybrid silica require "end-capping" to reduce "residual silanol groups" remaining on the silica surface after introducing the octadecyl group. Traditionally, surface treatment methods using methylsilane compounds have been employed.
However, from the perspective of reversed-phase separation, the silica surface ends up with two types of hydrophobic groups: those derived from ODS ligands and those from end-capping, which can result in uneven hydrophobic interactions. This can adversely affect peak shapes and theoretical plate numbers.
Instead of using classical methylsilane end-capping, such as trimethylsilyl groups (TMS), an innovative end-capping method using polar groups (ethers, hydroxyl groups) has been developed and applied to Cadenza CX-C18. The hydrophobic groups of solutes primarily interact with the octadecyl groups (ODS), while the hydrophilic groups interact electrostatically with the polar group end-capping structures.
As a result, improvements in peak shape and theoretical plate number for highly hydrophobic strong basic compounds are expected.
Additionally, the spatial localization of hydrophobic/hydrophilic groups on the stationary phase surface may enhance the recognition of solute stereostructures, potentially leading to separation characteristics distinct from traditional columns.
Cadenza CX-C18 with this novel end-capping method expands the world of versatile reversed-phase separation.